Application
Western blot
Sample
Human Tissue lysate - whole (stomach cancer tissue)
Loading amount
30 µg
Specification
stomach cancer tissue
Treatment
10uM cannabinoid antagonist
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 170 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 08 2013