Anti-EEF1D抗体(ab85964)
Key features and details
- Rabbit polyclonal to EEF1D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-EEF1D抗体
参阅全部 EEF1D 一抗 -
描述
兔多克隆抗体to EEF1D -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residues 130 and 180 of Human EEF1D (NP_001951.2)
-
阳性对照
- HeLa cell lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85964于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
|
|
IP |
Use at 2-5 - 5 µg/mg of lysate.
|
说明 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 32 kDa. |
IP
Use at 2-5 - 5 µg/mg of lysate. |
靶标
-
功能
Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE). -
组织特异性
Isoform 2 is specifically expressed in brain, cerebellum and testis. -
序列相似性
Belongs to the EF-1-beta/EF-1-delta family. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1936 Human
- Omim: 130592 Human
- SwissProt: P29692 Human
- Unigene: 333388 Human
- Unigene: 686554 Human
- Unigene: 703306 Human
-
别名
- Antigen NY CO 4 antibody
- Antigen NY-CO-4 antibody
- EEF 1D antibody
see all
图片
-
All lanes : Anti-EEF1D antibody (ab85964) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 3 seconds -
1mg HeLa whole cell lysate was immunoprecipitated using 3µg ab85964.
20% of IP was loaded per lane and probed with with ab85964 at 1 µg/ml (lane 1) and with a control IgG (lane 4).
Detection: chemiluminescence with an exposure time of 1 second.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab85964 尚未被引用在任何文献中。