Application
Western blot
Sample
Mouse Tissue lysate - whole (Pancreas or PDAC cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Pancreas or PDAC cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 150-100 kDa Non-specific: sometimes: 280 and 7 kDa
Positive control
#1 and #5-#9
Additional data
Additional Notes
#1-#4 and #10#11 = murine tissue
#5-#9 cell lysates
PubMed ID
28089234: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 14 2016