Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Loading amount
100000 cells
Specification
mouse embryonic stem cells
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti-rabbit IgG HRP linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ligia Tavares
Verified customer
提交于 Sep 16 2011