- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-E2F1抗体
参阅全部 E2F1 一抗 -
描述兔多克隆抗体to E2F1
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宿主Rabbit
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经测试应用适用于: IP, ICC/IF, WBmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human E2F1.
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阳性对照
- This antibody gave a positive signal in Human Small Intestine tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab112580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
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序列相似性Belongs to the E2F/DP family.
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翻译后修饰Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 535369 Cow
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- Unigene: 654393 Human
- Unigene: 18036 Mouse
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别名
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-E2F1 antibody (ab112580)ICC/IF image of ab112580 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112580 at 5µg/ml overnight at +4°C. The secondary antibody (green) was goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-E2F1 antibody (ab112580)All lanes : Anti-E2F1 antibody (ab112580) at 1 µg/ml
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276) with Immunizing peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesSecondary antibody - goat anti-rabbit HRP (H&L preadsorbed)
实验方案
文献
This product has been referenced in:
- Scott MC et al. Aberrant Retinoblastoma (RB)-E2F Transcriptional Regulation Defines Molecular Phenotypes of Osteosarcoma. J Biol Chem 290:28070-83 (2015). Read more (PubMed: 26378234) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"