Anti-E1 Ubiquitin Activating Enzyme 1/UBA1抗体(ab34711)
Key features and details
- Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1抗体
参阅全部 E1 Ubiquitin Activating Enzyme 1/UBA1 一抗 -
描述
兔多克隆抗体to E1 Ubiquitin Activating Enzyme 1/UBA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Dog -
免疫原
Fusion protein corresponding to Human E1 Ubiquitin Activating Enzyme 1/UBA1.
Database link: P22314 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
The product was purified from monospecific antiserum by a multi step procedure. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34711于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
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IHC-P |
Use a concentration of 2 - 20 µg/ml.
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). |
IHC-P
Use a concentration of 2 - 20 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. -
序列相似性
Belongs to the ubiquitin-activating E1 family. -
翻译后修饰
ISGylated. - Information by UniProt
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数据库链接
- Entrez Gene: 7317 Human
- Entrez Gene: 22201 Mouse
- Omim: 314370 Human
- SwissProt: P22314 Human
- SwissProt: Q02053 Mouse
- Unigene: 533273 Human
- Unigene: 1104 Mouse
- Unigene: 474674 Mouse
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别名
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
see all
图片
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All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Additional bands at: 35 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
This image shows human lung tissue stained with ab34711 at 10µg/ml. In many cells apunctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining.
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ICC/IF image of ab34711 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34711, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ab34711 (4µg/ml) staining E1 Ubiquitin activating enzyme in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and weak cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab34711 被引用在 8 文献中.
- Chen Z et al. Spermidine activates RIP1 deubiquitination to inhibit TNF-a-induced NF-?B/p65 signaling pathway in osteoarthritis. Cell Death Dis 11:503 (2020). PubMed: 32632306
- Shruthi K et al. Ubiquitin-proteasome system and ER stress in the brain of diabetic rats. J Cell Biochem 120:5962-5973 (2019). PubMed: 30317658
- Reddy SS et al. 4-PBA prevents diabetic muscle atrophy in rats by modulating ER stress response and ubiquitin-proteasome system. Chem Biol Interact 306:70-77 (2019). PubMed: 30980806
- Powis RA et al. Systemic restoration of UBA1 ameliorates disease in spinal muscular atrophy. JCI Insight 1:e87908 (2016). WB . PubMed: 27699224
- Park JH et al. PUGNAc induces protein ubiquitination in C2C12 myotube cells. Cell Biochem Funct 33:525-33 (2015). PubMed: 26531776
- Estrada-Bernal A et al. Functional complexity of the axonal growth cone: a proteomic analysis. PLoS One 7:e31858 (2012). WB, ICC/IF ; Rat . PubMed: 22384089
- Hjerpe R et al. Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes. Biochem J 441:927-36 (2012). WB . PubMed: 22004789
- Guinez C et al. Protein ubiquitination is modulated by O-GlcNAc glycosylation. FASEB J 22:2901-11 (2008). WB, IP ; Human . PubMed: 18434435