Anti-E. coli LPS抗体[2D7/1] (ab35654)
Key features and details
- Mouse monoclonal [2D7/1] to E. coli LPS
- Suitable for: ICC/IF, ELISA
- Reacts with: Escherichia coli
- Isotype: IgG2b
概述
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产品名称
Anti-E. coli LPS抗体[2D7/1] -
描述
小鼠单克隆抗体[2D7/1] to E. coli LPS -
宿主
Mouse -
特异性
The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.
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经测试应用
适用于: ICC/IF, ELISAmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Tissue, cells or virus corresponding to Escherichia coli E. coli LPS. Escherichia coli J5 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
2D7/1 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab35654于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (3) |
1/200.
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ELISA | (1) |
1/1500.
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说明 |
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ICC/IF
1/200. |
ELISA
1/1500. |
靶标
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相关性
Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock. -
别名
- Escherichia coli LPS antibody
图片
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ab35654 staining E. coli LPS in mouse hepatocytes (red). Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in SFX Kit blocking agent for 30 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 555 goat anti-mouse, diluted 1/100. Cells were stimulated for 90 minutes with E. coli LPS and then probed with ab35654. The picture was taken on a Leica confocal microscope that pseudo-colors the 555 secondary red. The blue staining was with To-Pro-III Iodide,again, pseudo-colored to be blue on confocal.
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab35654 被引用在 56 文献中.
- McGrath CJ et al. Development of a novel human intestinal model to elucidate the effect of anaerobic commensals on Escherichia coli infection. Dis Model Mech 15:N/A (2022). PubMed: 35302159
- Northrop-Albrecht EJ et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
- Fu A et al. Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer. Cell 185:1356-1372.e26 (2022). PubMed: 35395179
- Ma XY et al. Citation: Tight Junction Protein Expression-Inducing Probiotics Alleviate TNBS-Induced Cognitive Impairment with Colitis in Mice. Nutrients 14:N/A (2022). PubMed: 35889931
- Chen X et al. Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation. Nat Commun 13:7578 (2022). PubMed: 36481721