Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Swine testicle cells)
Permeabilization
Yes - Triton, 0.1%, 2min
Specification
Swine testicle cells
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: goat-anti-rabbit Texas Red
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Additional data
Additional Notes
No staining was observed, only a slight non-specific background. A secondary antibody only control was included (and yielded a similar picture). The fixative was 3% paraformaldehyde diluted in PBS and applied for 10min at room temperature.
Abcam response
Abcam is working with the reviewer to find an antibody more capable of detection of swine E cadherin.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 15 2015