Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Lung)
Permeabilization
Yes - 0.1% Tween 20 in PBS
Specification
Lung
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1/200 in TBS
Dilution
1/200
Additional data
Additional Notes
antigen retrieval was by heat mediation in citrate buffer pH 6.0
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Chia-Li Lin
Verified customer
提交于 Nov 20 2015