Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (7% Tris-Acetate Protein Gel in Tris-Acetate SDS Running Buffer)
Sample
Sheep Tissue lysate - whole (Left atrial appendage)
Specification
Left atrial appendage
Blocking step
SuperBlock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Bands
Specific: 250 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
Lane description:
1 - 30 ´g rat SM
2 - 30 ´g rat LV
3 - 10 ´g sheep RA
4 - 50 ´g sheep RA
5 - 100 ´g sheep RA
SM - skeletal muscle
LV - left ventricle
RA - left atrial appendage
Abcam response
We appreciate the valuable data provided in this Abreview. The antibody has been tested in sheep. The immunogen shows 91% identity with the sheep amino acid sequence of Dysferlin. However no positive results for sheep were obtained in the Western Blot shown in this Abreview.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 07 2014