Dynamin Inhibitors: Dynole™ Series试剂盒(ab120474)
概述
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产品名称
Dynamin Inhibitors: Dynole™ Series试剂盒 -
特异性
Convenient kit containing dynamin inhibitor Dynole® 34-2 (ab120463; 1 mg) and negative control Dynole® 31-2 (ab120464; 1mg) from the Dynole chemical series. Dynole® 34-2 targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in emzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold. -
产品概述
DynoleTM chemical series collection
Convenient kit containing dynamin inhibitor Dynole-34-2™ (ab120463; 1 mg) and negative control Dynole-31-2™ (ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.
Target
Description
GTPase Allosteric Site
Dynole-34-2™:
Potent dynamin I and dynamin II inhibitor
GTPase Allosteric Site
Dynole-31-2™:
Negative control for Dynole 34-2™
Convenient kit containing dynamin inhibitor Dynole-34-2™ ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.
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说明
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd. -
经测试应用
适用于: Functional Studiesmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 kit ab120464 - Dynole-31-2™ 1 x 1mg ab120463 - Dynole-34-2™ 1 x 1mg -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120474于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab120474 被引用在 6 文献中.
- Ferreira T et al. Oncolytic H-1 Parvovirus Enters Cancer Cells through Clathrin-Mediated Endocytosis. Viruses 12:N/A (2020). PubMed: 33096814
- Mayberry CL et al. JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by ß-Arrestin. J Virol 93:N/A (2019). PubMed: 30700597
- Stincardini C et al. An antipsychotic drug exerts anti-prion effects by altering the localization of the cellular prion protein. PLoS One 12:e0182589 (2017). PubMed: 28787011
- Albecka A et al. HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis. Traffic 17:21-39 (2016). PubMed: 26459807
- Schulze RJ et al. Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes. J Cell Biol 203:315-26 (2013). Functional Studies . PubMed: 24145164
- Hill TA et al. Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles. J Med Chem 52:3762-73 (2009). PubMed: 19459681