Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293T)
Loading amount
20 µg
Specification
HEK-293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Diluent: DPBS-tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Goat polyclonal to rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 100 kDa
Positive control
actin
Negative control
none
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 13 2007