Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Quail Tissue sections (Developing spinal cord)
Specification
Developing spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/750
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
This Ab should be diluted further.
Image shows ventral aspect of e6 developing spinal cord.
The central floorplate region is moderately positive.
However, longitudinal nerve fibres on both sides are intensely stained.
Cells/fibres within the ventricular area are also heavily stained
Image shows ventral aspect of e6 developing spinal cord.
The central floorplate region is moderately positive.
However, longitudinal nerve fibres on both sides are intensely stained.
Cells/fibres within the ventricular area are also heavily stained
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Apr 08 2011