Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain sections / Dentate Gyrus of the Hippocampus)
Antigen retrieval step
None
Permeabilization
Yes - PBST 3 x 5mn RT
Specification
Brain sections / Dentate Gyrus of the Hippocampus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Additional data
Additional Notes
Blue channel = GFAP
Red channel = ApoE
Green Channel = DCX
Doublecortin is expressed by mature neurons in the dentate gyrus i.e. granule cells. GFAP and ApoE are both expressed by Astrocytes, mainly present in the hilus and molecular layers of the DG. ApoE is also expressed by neural stem cells and GFAP is also expressed by immature neurons of the DG i.e. Type I Cells.
GFAP and ApoE staining both worked but DCX staining didn't work.
Abcam response
Thank you submitting your feedback using our Abreview. We will have the lab investigate this issue and are happy to replace or refund the antibody as per our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 01 2017