Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain: Coronal sections of striatum and cortex)
Specification
Brain: Coronal sections of striatum and cortex
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: vector lab Biotinylated Rabbit Anti-Sheep IgG Anti
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Additional Notes
Quenching step is suggested even though does not dramatically improve the staining. (I would advice not more than 10 min quenching).
Abcam response
Many thanks for your valuable feedback. Abcam has not validated this antibody in Immunohistochemistry on free floating sections as used in this Abreview. It appears that under these experimental conditions, ab49170 does only provide average results. However we would welcome further feedback of other researchers using ab19353.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 28 2016