Application
Western blot
Rat olfactory epithelium tissue lysate, 10ug/lane
Blocked overnight in 5% nonfat milk
GAP43 1/2000, 1hr in TBS+0.1%Tween
Donkey anti-sheep 1/5000 in 3.5%BSA/TBS+-.1%Tween
Lane1: GAP43 primary antibody, Lane 2: Secondary only negative control
There is a general high background to the secondary but seems to be improved with secondary incubation in BSA and extended washing after secondary incubation.
Blocked overnight in 5% nonfat milk
GAP43 1/2000, 1hr in TBS+0.1%Tween
Donkey anti-sheep 1/5000 in 3.5%BSA/TBS+-.1%Tween
Lane1: GAP43 primary antibody, Lane 2: Secondary only negative control
There is a general high background to the secondary but seems to be improved with secondary incubation in BSA and extended washing after secondary incubation.
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提交于 Jul 14 2015