驴抗兔IgG H&L (HRP) (ab97064)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
驴抗兔IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97064于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000.
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WB | (1) |
1/5000 - 1/50000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: Use 1/10000-1/50000
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说明 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. |
WB
1/5000 - 1/50000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: Use 1/10000-1/50000 |
图片
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ab19968 detecting vancomycin in human serum samples by indirect ELISA. Samples were blocked with 2.5% milk for 30 minutes at 20°C and incubated with the primary antibody (1/1000 in PBS) for 1 hour at 20°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97064) (1/1000) was used as the secondary antibody.
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Donkey polclonal secondary antibody to rabbit IgG-H&L (HRP) (ab97064) was used in a dilution of 1:5000. The first antibody was Smad4 antibody [EP618Y] (ab40759). The gel was a 4%-12% Bis-Tris Gel with MOPS Buffer. The first band is 30µg nuclear extract of a EBV-transformed B-lymphoblastoid cell line, the second band is 50µg of whole protein extract of the same EBV transformed B-LCL and the third lane is the same than in the second one but only 30µg. In this combination the secondary antibody works excellent. There is only the predicted band of 60 kDa, no addtional bands!
This image is courtesy of Mr Daniel Meier
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab97064 被引用在 31 文献中.
- Bhattarai U et al. IL-12α deficiency attenuates pressure overload-induced cardiac inflammation, hypertrophy, dysfunction, and heart failure progression. Front Immunol 14:1105664 (2023). PubMed: 36860846
- Otte K et al. Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide. Biomedicines 10:N/A (2022). PubMed: 36140245
- Leader Y et al. The upstream 5' splice site remains associated to the transcription machinery during intron synthesis. Nat Commun 12:4545 (2021). PubMed: 34315864
- Pirozzi F et al. Proximal variants in CCND2 associated with microcephaly, short stature, and developmental delay: A case series and review of inverse brain growth phenotypes. Am J Med Genet A 185:2719-2738 (2021). PubMed: 34087052
- Zhao K et al. Inhibition of Carbonic Anhydrase 2 Overcomes Temozolomide Resistance in Glioblastoma Cells. Int J Mol Sci 23:N/A (2021). PubMed: 35008590