驴抗兔IgG H&L (Alkaline Phosphatase) (ab97061)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, ELISA, WB, IHC-P
概述
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产品名称
驴抗兔IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
经测试应用
适用于: ICC, ELISA, WB, IHC-Pmore details -
偶联物
Alkaline Phosphatase
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97061于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent dilution.
|
|
ELISA |
1/5000 - 1/50000.
|
|
WB |
1/5000 - 1/50000.
|
|
IHC-P |
1/100 - 1/1000.
|
说明 |
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ICC
Use at an assay dependent dilution. |
ELISA
1/5000 - 1/50000. |
WB
1/5000 - 1/50000. |
IHC-P
1/100 - 1/1000. |
图片
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All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution
Lanes 3-4 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution
Lanes 5-6 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (10)
ab97061 被引用在 10 文献中.
- Deshmukh A et al. Analytical sameness methodology for the evaluation of structural, physicochemical, and biological characteristics of Armlupeg: A pegfilgrastim biosimilar case study. PLoS One 18:e0289745 (2023). PubMed: 37556495
- Chang KT et al. Aberrant cyclin C nuclear release induces mitochondrial fragmentation and dysfunction in MED13L syndrome fibroblasts. iScience 25:103823 (2022). PubMed: 35198885
- Willis SD et al. Cyclin C-Cdk8 Kinase Phosphorylation of Rim15 Prevents the Aberrant Activation of Stress Response Genes. Front Cell Dev Biol 10:867257 (2022). PubMed: 35433688
- Heidler TV et al. Porphyromonas gingivalis fimbrial protein Mfa5 contains a von Willebrand factor domain and an intramolecular isopeptide. Commun Biol 4:106 (2021). PubMed: 33495563
- Hanley SE et al. Snx4-assisted vacuolar targeting of transcription factors defines a new autophagy pathway for controlling ATG expression. Autophagy 17:3547-3565 (2021). PubMed: 33678121
- Jezek J et al. Synergistic repression of thyroid hyperplasia by cyclin C and Pten. J Cell Sci 132:N/A (2019). PubMed: 31331961
- Jezek J et al. Mitochondrial translocation of cyclin C stimulates intrinsic apoptosis through Bax recruitment. EMBO Rep 20:e47425 (2019). PubMed: 31385392
- Molina-Aguilar C et al. Time-caloric restriction inhibits the neoplastic transformation of cirrhotic liver in rats treated with diethylnitrosamine. Carcinogenesis 38:847-858 (2017). PubMed: 28535183
- Kilinc S et al. Lysine-specific demethylase-1 (LSD1) is compartmentalized at nuclear chromocenters in early post-mitotic cells of the olfactory sensory neuronal lineage. Mol Cell Neurosci 74:58-70 (2016). PubMed: 26947098
- Mehrbod P et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). WB . PubMed: 24788303