驴Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

概述

  • 产品名称
    驴Anti-Goat IgG H&L (Alexa Fluor® 488)
    参阅全部 IgG 二抗
  • 描述
    驴多克隆抗体二抗to山羊IgG - H&L (Alexa Fluor® 488)
  • 靶标种属
    Goat
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • 偶联物
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab150129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

图片

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H+L) (shown in green) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Overlay histogram showing Jurkat cells stained Goat polyclonal to Ikaros (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Goat polyclonal to Ikaros, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 donkey anti-goat IgG (H&L) (ab150129) at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was goat IgG (polyclonal) (ab37373, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

文献

This product has been referenced in:
  • Telegina DV  et al. Contributions of age-related alterations of the retinal pigment epithelium and of glia to the AMD-like pathology in OXYS rats. Sci Rep 7:41533 (2017). ICC/IF ; Rat . Read more (PubMed: 28134357) »
  • Liu Y  et al. TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus. Front Immunol 8:651 (2017). Flow Cyt ; Human . Read more (PubMed: 28620393) »

See all 28 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA fixed)
Very good antibody
Username

Yacine Tensaouti

Verified customer

提交于 Aug 01 2017

For excitation with the 543nm laser, Alexa Fluor 568 is a better choice than 555 for combination with AF488. There is still some emission spillover but it is much less than for the 488/555 combination, and you should be able to set compensation without...

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Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab150129. I would also appreciate if you can confirm some further details: Do you use a detergent such as Tween 20 in your washing buffer? Have you a...

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Application
Immunocytochemistry/ Immunofluorescence
Following fixation in 4% PFA, the cells were assessed for Brachyury (T) expression using 1:100 dilution of the primary antibody in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using donkey polyclonal rabbit IgG Alexa 488 (ab150129) at 1:500. The results show that nuclear T (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here). All nuclei were counterstained using DAPI (blue).
Username

Abcam user community

Verified customer

提交于 Sep 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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