Application
Western blot
Sample
Human Cell lysate - whole cell (Human HaCaT keratinocytes)
Loading amount
30 µg
Specification
Human HaCaT keratinocytes
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
LiCor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: Odyssey Blocking Buffer + 0.1% Tween20
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IRDye 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Dilution
1/20000
Detection
Detection method
Odyssey infra red
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ? kDa Non-specific: >250, <70 (multiple) kDa
Positive control
Cells confirmed to express Dock% by mass spectrometry
Additional data
Abcam response
We would like to thank the reviewer for submitting this Abreview for product ab71578 (Anti-Dock5 antibody). As this product had not yet been tested in Western Blot on Human HaCaT keratinocytes we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests. We wish to thank them for participating in our 100% Abreview testing discount program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Leeni Koivisto
Verified customer
提交于 Jun 20 2012