Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/500
Additional data
Abcam response
Thank you for sharing your data with us. ab2850 has not previously been tested on marmoset and we are currently unsure of the correct staining pattern for Dnmt3a in the marmoset testis. Abcam would welcome feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 煜欽 林
Verified customer
提交于 Jan 21 2014