Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HCT116)
Specification
HCT116
Total protein in input
50 µg
Other product details
Concentration
5 µg/mg lysate
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP Buffer (Tris, Triton, EDTA, NaCl)
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
Mass Spectrometry was performed to identify the proteins present. All the peptides obtained were checked against peptides obtained from a no antibody control lysate and other non-related protein.
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提交于 Mar 03 2014