Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Human Cell lysate - nuclear (HCT116)
Specification
HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.5% Tween + Azide
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Other
Host species: Mouse
Clonality: Polyclonal
Conjugation: Other
Dilution
1/5000
Detection
Detection method
Odyssey machine
Bands
Specific: 185 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
The lower bands were not observed, because the gel was allowed to run so that the protein 50KDa and lower escaped, for better resolution of bigger proteins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 23 2013