Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Sample
Human Cell lysate - whole cell (HCT116)
Specification
HCT116
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + Azid
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/40000
Detection
Detection method
ECL
Bands
Specific: 183 kDa Non-specific: many kDa
Exposure
10 second(s)
Additional data
Additional Notes
lane 1 (left) - 20ug protein loaded.
lane 2 (middle) - 40ug protein loaded.
lane 3 (right) - 60ug protein loaded.
top marker line - ~250kDa
lower marker line - ~130kDa
Abcam response
We are sorry that this antibody did not provide satisfactory results for the customer.
The antibody is covered by our Abpromise guarantee in WB and human. Customer was contacted in order to discuss the results and confirmed that other antibody dilutions (such as 1:4000 and possibly 1:5000) provided better results (less background and a clear main band at the expected molecular weight).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 03 2014