Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (hek 293)
Specification
hek 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 0.1% TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Li-CoR Donkey anti Mouse Antibody
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye 800CW
Dilution
1/15000
Detection
Detection method
Li-Cor Odyssey Scanner
Bands
Specific: 180, 140 250 kDa
Exposure
10 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 02 2014