Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Blocking step
Blocking reagent (Roche) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: water
Secondary antibody
Name
Non-Abcam antibody was used: anti goat HRP polymer
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
application: ISH (didn't found the symbol) but I think it will work for IHC-P also.
working concentration: 1/1000-1/6000
2nd antibody: N/A
working concentration: 1/1000-1/6000
2nd antibody: N/A
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 06 2012