Application
Western blot
Sample
Hamster Cell lysate - whole cell (VeroE6 (Kidney from African green monkey), BHK21 ()
Gel Running Conditions
Reduced Denaturing (4-12% NuPage gel, MES buffer)
Loading amount
25000 cells
Specification
VeroE6 (Kidney from African green monkey), BHK21 (
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% milk in PBS/Tween20(0.01%)
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-Mouse IgG (whole molecule)–Peroxidase antibod
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity Western ECL substrate (170-5061,
Exposure
45 second(s)
Positive control
Positive control was a lysate of A549 cells. Bands were also detected in VeroE6 cells. No bands were detected in BHK21 and MRK101 cell lines.
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提交于 Jul 11 2016