Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Rat Tissue lysate - whole (Mammary Tumor)
Specification
Mammary Tumor
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
68 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse Antibody
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 250 kDa Non-specific: 45, 60 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Antibody sequence has high homology with Rat per Abcam service Rep.
PVDF membrane was used.
Probed with secondary overnight at 4 degrees.
Promotion code associated with this review: ABTRIAL-TBDU6
Abcam response
Thank you very much for your feedback.
For users wishing to test our products in untested species or applications, we would encourage them to participate in our Abtrial program, by which you will beneficiate from a discount off your next purchase for the value of the product tested by submitting your results to our website.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
We would welcome more feedback from our customers in order to make further decision about the suitability of this antibody with rat species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 30 2013