Anti-Detyrosinated alpha Tubulin抗体- Microtubule Marker (ab48389)
Key features and details
- Rabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Detyrosinated alpha Tubulin抗体- Microtubule Marker
参阅全部 Detyrosinated alpha Tubulin 一抗 -
描述
兔多克隆抗体to Detyrosinated alpha Tubulin - Microtubule Marker -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.
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常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48389于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (6) |
1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).
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说明 |
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WB
1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa). |
靶标
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相关性
Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Omim: 191110 Human
- Omim: 602528 Human
- SwissProt: P68366 Human
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别名
- Alpha tubulin 1 antibody
- TUBA1 antibody
- TUBA2 antibody
see all
图片
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All lanes : Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) at 1/500 dilution
Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Detyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (77)
ab48389 被引用在 77 文献中.
- Rafiq NM et al. JIP3 links lysosome transport to regulation of multiple components of the axonal cytoskeleton. Commun Biol 5:5 (2022). PubMed: 35013510
- Moriarty RA et al. RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration. iScience 25:103845 (2022). PubMed: 35198898
- Halpern AR et al. Versatile, do-it-yourself, low-cost spinning disk confocal microscope. Biomed Opt Express 13:1102-1120 (2022). PubMed: 35284165
- Tavares S et al. FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancer. Cell Rep 39:110584 (2022). PubMed: 35385742
- Phyo SA et al. Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure. Front Cell Dev Biol 10:837486 (2022). PubMed: 35433678