Anti-Desmoglein 2/DSG2抗体(ab96761)
Key features and details
- Rabbit polyclonal to Desmoglein 2/DSG2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Desmoglein 2/DSG2抗体
参阅全部 Desmoglein 2/DSG2 一抗 -
描述
兔多克隆抗体to Desmoglein 2/DSG2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Desmoglein 2/DSG2 aa 1-176.
Database link: NP_001934 -
阳性对照
- WB: 293T, H1299 and MOLT4 whole cell lysates; HepG2 cell lysate; HeLa cell lysate. IHC-P: DU145 xenograft ICC/IF: A431 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
|
IHC-P |
1/100 - 1/500.
|
说明 |
---|
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa. Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
IHC-P
1/100 - 1/500. |
靶标
-
功能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
组织特异性
All of the tissues tested and carcinomas. -
疾病相关
Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Contains 4 cadherin domains. -
结构域
Calcium may be bound by the cadherin-like repeats. -
细胞定位
Cell membrane. Cell junction > desmosome. - Information by UniProt
-
数据库链接
- Entrez Gene: 1829 Human
- Entrez Gene: 13511 Mouse
- Omim: 125671 Human
- SwissProt: Q14126 Human
- SwissProt: O55111 Mouse
- Unigene: 412597 Human
- Unigene: 345891 Mouse
-
别名
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
see all
图片
-
Paraffin-embedded mouse skin labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0) 15 minutes.
-
Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
-
Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 30 µg
Secondary
Anti-Rabbit IgG HRP
Predicted band size: 122 kDa -
All lanes : Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa
7.5% SDS Page -
Immunohistochemical analysis of Desmoglein 2/DSG2 in paraffin embedded DU145 (Human) xenograft, using ab96761 at a 1/100 dilution.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab96761 尚未被引用在任何文献中。