Anti-Desmoglein 1/DSG1抗体[27B2] (ab12077)
Key features and details
- Mouse monoclonal [27B2] to Desmoglein 1/DSG1
- Suitable for: IHC-Fr
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Desmoglein 1/DSG1抗体[27B2]
参阅全部 Desmoglein 1/DSG1 一抗 -
描述
小鼠单克隆抗体[27B2] to Desmoglein 1/DSG1 -
宿主
Mouse -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-Fr: Human normal skin.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
27B2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12077于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-Fr
Use a concentration of 1 µg/ml. |
靶标
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功能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
组织特异性
Epidermis, tongue, tonsil and esophagus. -
疾病相关
Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger. -
序列相似性
Contains 4 cadherin domains. -
结构域
Calcium may be bound by the cadherin-like repeats. -
细胞定位
Cell membrane. Cell junction > desmosome. - Information by UniProt
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数据库链接
- Entrez Gene: 1828 Human
- Omim: 125670 Human
- SwissProt: Q02413 Human
- Unigene: 2633 Human
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别名
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
see all
图片
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IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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ab12077 staining Desmoglein 1/DSG1 in human skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab12077 被引用在 5 文献中.
- Simpson CL et al. NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell Rep 34:108689 (2021). PubMed: 33535046
- Yuan J et al. SHROOM2 inhibits tumor metastasis through RhoA-ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death Dis 10:58 (2019). PubMed: 30683844
- Tsutsumi M et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . PubMed: 19657674
- Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . PubMed: 17971852
- Wu H et al. Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation. J Invest Dermatol 120:1052-7 (2003). PubMed: 12787134