Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Loading amount
50 µg
Specification
hek293
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST+5% milk
Secondary antibody
Name
Non-Abcam antibody was used: mouse anti rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 26 2010