Anti-Dengue Virus prM glycoprotein抗体[DM1] (ab41473)
Key features and details
- Mouse monoclonal [DM1] to Dengue Virus prM glycoprotein
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Dengue virus 2
- Isotype: IgG1
概述
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产品名称
Anti-Dengue Virus prM glycoprotein抗体[DM1]
参阅全部 Dengue Virus prM glycoprotein 一抗 -
描述
小鼠单克隆抗体[DM1] to Dengue Virus prM glycoprotein -
宿主
Mouse -
特异性
This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4. -
经测试应用
适用于: ICC/IF, WB, ELISAmore details -
种属反应性
与反应: Dengue virus 2 -
免疫原
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
DM1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41473于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/5 - 1/20.
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WB |
1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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ELISA |
Use at an assay dependent dilution.
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说明 |
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ICC/IF
1/5 - 1/20. |
WB
1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ELISA
Use at an assay dependent dilution. |
靶标
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相关性
There are three structural proteins found in Dengue virus - E glycoprotein, anchored capsid (anC) and pre-membrane (prM). prM cleavage occurs in the final stages of virion maturation. This results in the dissociation of prM-E glycoprotein dimers which allows E glycoprotein to mediate fusion with the host cell membrane. -
别名
- pre membrane glycoprotein antibody
- pre-membrane glycoprotein antibody
图片
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All lanes : Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)
Lanes 1-2 : Dengue Virus 1 infected C6/36 cell lysate
Lanes 3-4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate
Lanes 5-6 : Dengue Virus 2 (16681) infected C6/36 cell lysate
Lanes 7-8 : Dengue Virus 3 infected C6/36 cell lysate
Lanes 9-10 : Dengue Virus 4 infected C6/36 cell lysate
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab41473 被引用在 9 文献中.
- Jiang L & Sun Q The Role of Autophagy-Mediated Dengue Virus Antibody-Dependent Enhancement Infection of THP-1 Cells. Intervirology 63:57-65 (2020). PubMed: 33202415
- Cortese M et al. Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production. Cell Rep 27:2579-2592.e6 (2019). PubMed: 31141684
- Xie X et al. Dengue NS2A Protein Orchestrates Virus Assembly. Cell Host Microbe 26:606-622.e8 (2019). PubMed: 31631053
- Saisawang C et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13:e0193133 (2018). PubMed: 29470500
- Jiang L & Sun Q The expression profile of human peripheral blood mononuclear cell miRNA is altered by antibody-dependent enhancement of infection with dengue virus serotype 3. Virol J 15:50 (2018). PubMed: 29566761
- Huang X et al. Antibody-dependent enhancement of dengue virus infection inhibits RLR-mediated Type-I IFN-independent signalling through upregulation of cellular autophagy. Sci Rep 6:22303 (2016). PubMed: 26923481
- Palma-Ocampo HK et al. Interferon lambda inhibits dengue virus replication in epithelial cells. Virol J 12:150 (2015). ICC/IF . PubMed: 26411318
- Décembre E et al. Sensing of immature particles produced by dengue virus infected cells induces an antiviral response by plasmacytoid dendritic cells. PLoS Pathog 10:e1004434 (2014). PubMed: 25340500
- Liang Z et al. Activation of Toll-like receptor 3 impairs the dengue virus serotype 2 replication through induction of IFN-ß in cultured hepatoma cells. PLoS One 6:e23346 (2011). ICC/IF ; Dengue virus 2 . PubMed: 21829730