Application
Flow Cytometry
Fixation
BD CytoFix/CytoPerm
Permeabilization
Yes - BD CytoFix/CytoPerm
Sample
Human Cell (HeLa at 62hpi with DV2)
Specification
HeLa at 62hpi with DV2
Gating Strategy
single cells by FSC and SSC
Preparation
Cell harvesting/tissue preparation method: spatula scraping
Sample buffer: cold PBS
Sample buffer: cold PBS
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: BD CytoPerm/CytoWash
Dilution
1/80
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen A21121 Gt anti-MsIgG1 AlexaFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/150
Additional data
Additional Notes
At 1:80, this primary antibody detected a 1.5-log signal in DV2-infected HeLa cells. The antibody agreed with our other anti-DV antibodies, identifying about 30% Dengue-positive cells in this experiment.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Lee Gehrke
Verified customer
提交于 Oct 07 2013