Anti-DeltaC抗体[zdc2] (ab73336)
Key features and details
- Mouse monoclonal [zdc2] to DeltaC
- Suitable for: ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, WB
- Reacts with: Zebrafish
- Isotype: IgG2a
概述
-
产品名称
Anti-DeltaC抗体[zdc2] -
描述
小鼠单克隆抗体[zdc2] to DeltaC -
宿主
Mouse -
特异性
Delta proteins activate Notch through a binding reaction that depends on their extracellular domains. During somitogenesis, Notch signalling via DeltaC keeps the oscillations of neighbouring cells in synchrony. DeltaC is also expressed in subsets of cells in the nervous system and elsewhere. -
经测试应用
适用于: ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, WBmore details -
种属反应性
与反应: Zebrafish -
免疫原
Extracellular region of DeltaC protein (Zebrafish).
-
表位
DeltaC extracellular domain. -
阳性对照
- HEK293 cells transfected with DeltaC or zebrafish embryo cryosections (formulin fixed).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
zdc2 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73336于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IHC - Wholemount | (1) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration.
|
说明 |
---|
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC - Wholemount
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
-
相关性
DeltaC acts as a ligand for Notch receptors and is involved in somitogenesis. It can activate Notch receptors. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized. -
细胞定位
Membrane; Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 30120 Zebrafish
- SwissProt: Q9IAT6 Zebrafish
-
别名
- Bea antibody
- Beamter antibody
- DelC antibody
- Dlc antibody
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab73336 被引用在 5 文献中.
- Sehgal P et al. LncRNA VEAL2 regulates PRKCB2 to modulate endothelial permeability in diabetic retinopathy. EMBO J 40:e107134 (2021). PubMed: 34180064
- Tietz KT et al. Pumilio response and AU-rich elements drive rapid decay of Pnrc2-regulated cyclic gene transcripts. Dev Biol 462:129-140 (2020). PubMed: 32246943
- Gallagher TL et al. Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Dev Biol 429:225-239 (2017). PubMed: 28648842
- Quillien A et al. Distinct Notch signaling outputs pattern the developing arterial system. Development 141:1544-52 (2014). Zebrafish . PubMed: 24598161
- Giudicelli F et al. Setting the tempo in development: an investigation of the zebrafish somite clock mechanism. PLoS Biol 5:e150 (2007). PubMed: 17535112