Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (telencephalons)
Specification
telencephalons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% milk
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: ECL HRP linked donkey anti-rabbit
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Luminata
Bands
Specific: none kDa Non-specific: 110 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
E15 telencephalon Lysate.
Additional data
Additional Notes
Having seen no band for Dab1 from my telencephalon lysate, with this antibody, I reprobed the blot with an antibody to the C-terminal portion of Dab1 and got a nice strong band.
This anybody did however detect Dab1 in a lysate from HEK293Tcells transfected with Dab1.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 27 2014