Anti-DYRK1A抗体(ab65220)
Key features and details
- Rabbit polyclonal to DYRK1A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DYRK1A抗体
参阅全部 DYRK1A 一抗 -
描述
兔多克隆抗体to DYRK1A -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human DYRK1A aa 21-70 (internal sequence).
Database link: Q13627 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab65220 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65220于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/500 - 1/1000.
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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说明 |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
靶标
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功能
May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates. -
组织特异性
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. -
疾病相关
Defects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
发展阶段
Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain. -
结构域
The polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028). -
翻译后修饰
Autophosphorylated on tyrosine residues. -
细胞定位
Nucleus speckle. - Information by UniProt
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数据库链接
- Entrez Gene: 1859 Human
- Omim: 600855 Human
- SwissProt: Q13627 Human
- Unigene: 368240 Human
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别名
- Dual specificity tyrosine phosphorylation regulated kinase antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
see all
图片
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ab65220, at a 1/500 dilution, staining DYRK1A in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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All lanes : Anti-DYRK1A antibody (ab65220) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 86 kDa
Observed band size: 86 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab65220 被引用在 2 文献中.
- Fujita K et al. Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo. Int J Oncol 46:2419-30 (2015). PubMed: 25823465
- Baek KH et al. Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR1. Nature 459:1126-30 (2009). WB ; Human . PubMed: 19458618