Application
Western blot
Sample
Rat Recombinant protein (Recombinant protein cloned into EcoR1)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine Gel)
Loading amount
0.5 µg
Specification
Recombinant protein cloned into EcoR1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-Tween + 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRdye 680 donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Detection
Detection method
Licor Odyssey
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 11 kDa Non-specific: 37 kDa
Additional data
Additional Notes
First lane: ladder from the bottom up: 10, 15, 20 kD. Second lane: LC8 recombinant protein, which runs at a slightly higher molecular weight than 8 kD when run on a tris-glycine gel.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 07 2010