Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord homogenate)
Gel Running Conditions
Reduced Denaturing (10% SDS_PAGE)
Loading amount
20 µg
Treatment
sham or nerve transected
Specification
spinal cord homogenate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Santa Cruz Donkey anti-Goat-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Sham
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 40 kD kDa Non-specific: 83, 195 kDa
Positive control
Nerve transected
Additional data
Additional Notes
I would dilute this antibody out more, maybe 1:1000 or even 1:5000 to get rid of the non-specific bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 17 2008