Anti-DUOX2抗体(ab97266)
Key features and details
- Rabbit polyclonal to DUOX2
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-DUOX2抗体 -
描述
兔多克隆抗体to DUOX2 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat
预测可用于: Human, Chimpanzee, Macaque monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Rat DUOX2 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: Q9ES45 -
阳性对照
- This antibody gave a positive signal in Rat Colon tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Rat Thyroid.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97266于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 175 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 175 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain. -
组织特异性
Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis. -
通路
Hormone biosynthesis; thyroid hormone biosynthesis. -
疾病相关
Defects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6) [MIM:607200]. A disorder due to a defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
序列相似性
In the N-terminal section; belongs to the peroxidase family.
Contains 3 EF-hand domains.
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
发展阶段
Widely expressed in fetal tissues. -
翻译后修饰
N-glycosylated. -
细胞定位
Apical cell membrane. Localizes to the apical membrane of epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 50506 Human
- Entrez Gene: 79107 Rat
- Omim: 606759 Human
- SwissProt: Q9NRD8 Human
- SwissProt: Q9ES45 Rat
- Unigene: 71377 Human
- Unigene: 55542 Rat
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别名
- Dual oxidase 2 antibody
- Dual oxidase like domains 2 antibody
- Dual oxidase2 antibody
see all
图片
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IHC image of DUOX2 staining in Rat thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97266, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Anti-DUOX2 antibody (ab97266) at 1 µg/ml (Milk blocking (3%)) + Colon (Rat) Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 175 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab97266 被引用在 5 文献中.
- Lyu PW et al. Overexpression of DUOX2 mediates doxorubicin resistance and predicts prognosis of pancreatic cancer. Gland Surg 11:115-124 (2022). PubMed: 35242674
- Yang M et al. A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis. Cancer Biol Med 18:215-226 (2021). PubMed: 33628596
- Chen J et al. Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells. Signal Transduct Target Ther 5:139 (2020). PubMed: 32792487
- Cao L et al. DUOX2, a common modulator in preventive effects of monoamine-based antidepressants on water immersion restraint stress- and indomethacin- induced gastric mucosal damage. Eur J Pharmacol 876:173058 (2020). PubMed: 32131022
- Dho SH et al. NOX5-L can stimulate proliferation and apoptosis depending on its levels and cellular context, determining cancer cell susceptibility to cisplatin. Oncotarget 6:39235-46 (2015). PubMed: 26513170