Application
Western blot
Sample
Bird Tissue lysate - whole (Pectoralis muscle)
Gel Running Conditions
Reduced Denaturing (7.5% acrylamide)
Loading amount
20 µg
Treatment
Homogenized in Tris (5mM) / EGTA (2mM) solution
Specification
Pectoralis muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 5%
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: A4416 SIGMA / Anti-Mouse IgG (whole molecule)–Pero
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 82 kDa
Positive control
Rat soleus muscle. Whole tissue lysate. Homogenized in Tris (5mM) / EGTA (2mM) solution. Loading amount : 20 µg
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提交于 Oct 10 2016