Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Total protein in input
1e+007 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
A549 (human lung epithelium cells)
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce™ IP Lysis Buffer
Dilution
1/250
Western Blot antibody
Name
Abcam antibody:Anti-DRP1 antibody
Additional data
Additional Notes
Lane 1: IP by control mouse IgG
Lane 2: IP by DRP1 antibody (Abcam ab56788)
Band corresponding to DRP1 (75 kDa) can be detected in DRP1 pulldown sample but not control IgG sample. Non-specific bands (43 kDa) also shown in the pulldown sample. Heavy chain and light chain of IP antibodies can be seen around 50 kDa and 25 kDa.
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提交于 Jul 12 2016