Anti-DR1抗体(ab88597)
Key features and details
- Mouse polyclonal to DR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-DR1抗体
参阅全部 DR1 一抗 -
描述
小鼠多克隆抗体to DR1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein within Human DR1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: PC12, Raw 264.7, Jurkat, NIH/3T3 and HeLa nuclear cell lysates; DR1 transfected 293T cell lysate; ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88597于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
序列相似性
Belongs to the NC2 beta/DR1 family.
Contains 1 histone-fold domain. -
翻译后修饰
Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1810 Human
- Entrez Gene: 13486 Mouse
- Entrez Gene: 289881 Rat
- Omim: 601482 Human
- SwissProt: Q01658 Human
- SwissProt: Q91WV0 Mouse
- SwissProt: Q5XI68 Rat
- Unigene: 348418 Human
see all -
别名
- Down regulator of transcription 1 antibody
- Down regulator of transcription 1 TBP binding antibody
- Down-regulator of transcription 1 antibody
see all
图片
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Anti-DR1 antibody (ab88597) at 1 µg/ml + PC12 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands. -
Anti-DR1 antibody (ab88597) at 1 µg/ml + HeLa nuclear cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Anti-DR1 antibody (ab88597) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Anti-DR1 antibody (ab88597) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
Anti-DR1 antibody (ab88597) at 1 µg/ml + NIH3T3 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
All lanes : Anti-DR1 antibody (ab88597) at 1 µg/ml
Lane 1 : DR1 transfected 293T lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
ab88597 at 10µg/ml staining DR1 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab88597 被引用在 3 文献中.
- Wang T et al. Antiepileptic effect of uridine may be caused by regulating dopamine release and receptor expression in corpus striatum. Brain Res 1688:47-53 (2018). PubMed: 29555238
- Hsu SF et al. A host susceptibility gene, DR1, facilitates influenza A virus replication by suppressing host innate immunity and enhancing viral RNA replication. J Virol 89:3671-82 (2015). PubMed: 25589657
- Sun Z et al. Functional divergence of the rapidly evolving miR-513 subfamily in primates. BMC Evol Biol 13:255 (2013). WB ; Human . PubMed: 24252134