Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Jurkat cells)
Total protein in input
2e+007 cells
Specification
Jurkat cells
Immuno-precipitation step
Protein A
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s)
Western Blot antibody
Name
Non-Abcam antibody was used: mouse monoclonal HRP
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
In fact, I have done an IP using A protein coated beads, I put ab8112 (1ug/ml) during about 2 hrs at room temperature under shaking ... Then I put beads with lysates for 2 hrs at 4 oC on a wheel . Next gel migration and transfer followed by a blocking step of the membrane in PBS BSA 5% (1hr RT or overnight (8 hrs minimum) at 4 oC) under shaking. I used a first ab 4G10 to check Y phosphorylations and a secondary ab monoclonal mouse HRP coupled, then ECL and blotting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Guittard Geoffrey
Verified customer
提交于 Jul 27 2006