Application
Western blot
Sample
Human Cell lysate - whole cell (Tumor cells)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
30 µg
Specification
Tumor cells
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: HRP-labeld anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 0 kDa Non-specific: 130, 80, 70, 40 kDa
Additional data
Additional Notes
Very high background and No specific polymerase gamma band was found,
Abcam response
Thank you for your valuable feedback.
We would recommend running the gel in reducing conditions, blocking with 3% BSA, and incubating the antibody for 1 hour at room temperature to improve the results.
Since the results are not satisfactory, we will offer a replacement or credit in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Prof. Yongliang Zhao
Verified customer
提交于 Nov 22 2011