Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Small Cell Lung Cancer)
Specification
Small Cell Lung Cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Bond ER1
Permeabilization
No
Other product details
Dilution
1/200
Incubation time
15 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Vector labs polymer kit
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Although I optimised the staining manually the best results were using the BondMax autostainer with a polymer secondary and DABe. ER1 is equivalent to citrate buffer. Obviously on the BondMax I used their polymer system.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 18 2009