DNA Damage Kinases组合(ATM,ATM pS1981,ATR,ATR pS428,DNA-PKcs,DNA-PKcs pS2056) (ab103970)
概述
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产品名称
DNA Damage Kinases组合(ATM,ATM pS1981,ATR,ATR pS428,DNA-PKcs,DNA-PKcs pS2056) -
产品概述
ab103970 is a DNA Damage Kinases Panel designed for the validation and characterization of the phosphorylation state of the DNA damage responsive kinases ATM and DNA-PKcs. ATM and DNA-PK are protein kinases that are active and become phosphorylated in response to DNA double strand breaks (DSBs). ATM is phosphorylated on S1981 and phosphorylates various proteins during the cellular response to DNA DSBs, while DNA-PK, a key component of the non-homologous end-joining pathway of DSB repair, is phosphorylated on S2056.
February 20th 2019: Please note these changes: The following changes were made to this panel: Anti-ATM antibody (ab82512) was replaced with Anti-ATM antibody [EPR17059] (ab199726), Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192) was replaced with Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) and Anti-DNA PKcs antibody (ab70250) was replaced with Anti-DNA PKcs antibody [Y393] (ab32566).
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说明
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
研究领域
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别名
- A-T mutated
- A-T mutated homolog
- Ataxia telangiectasia mutated
see all
相关产品
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Related Products
- Anti-DNA PKcs antibody [Y393] - BSA and Azide free (ab174575)
- Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - BSA and Azide free (ab174576)
- HRP Anti-DNA PKcs antibody [Y393] (ab195537)
- Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
- Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)
图片
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All lanes : Anti-ATM antibody (ab82512) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ab82512 was raised against an immunogen that is predicted to cross react with both isoform 1 (350 kDa) and isoform 2 (195 kDa) of Human Serine-protein kinase ATM (ATM). We believe that the band observed at 195 kDa corresponds to isoform 2. -
All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Extract from Patient with Ataxia-Telangiectasia Whole Cell Lysate
Lane 3 : Irradiated HeLa Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Additional bands at: 100 kDa, 110 kDa, 145 kDa, 200 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-DNA PKcs antibody (ab70250) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 15 µg
Lane 2 : Whole cell lysate from Hela cells at 50 µg
Additional bands at: 170 kDa, 180 kDa, 230 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-DNA PKcs (phospho S2056) antibody (ab18192) at 1 µg/ml
Lane 1 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823)
Lane 2 : Untreated HeLa cell extract
Lane 3 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
Lane 4 : Untreated HeLa cell extract with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
Lane 5 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) with Human DNA PKcs peptide (ab20407) at 1 µg/ml
Lane 6 : Untreated HeLa cell extract with Human DNA PKcs peptide (ab20407) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
ab18192 specifically recognizes a band at ~460 kDa corresponding to DNA PKcs in HeLa cells that have been treated with ionizing radiation (lane 1). This band is not detected in untreated cells (lane 2). The activity of the antibody is quenched by the addition of the immunizing (modified) peptide, ab20406 (lanes 3) but not the unmodified peptide, ab20407 (lane 5). For the ab13823 irradiated HeLa cell lysate, the 4 hour post-treatment extract was used.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab103970 被引用在 4 文献中.
- Yang X et al. High Expression of Cancer-IgG Is Associated With Poor Prognosis and Radioresistance via PI3K/AKT/DNA-PKcs Pathway Regulation in Lung Adenocarcinoma. Front Oncol 11:675397 (2021). PubMed: 34150640
- Ma YF et al. Nuclear factor erythroid 2-related factor 2-antioxidant activation through the action of ataxia telangiectasia-mutated serine/threonine kinase is essential to counteract oxidative stress in bovine mammary epithelial cells. J Dairy Sci 101:5317-5328 (2018). PubMed: 29605311
- Velkova A et al. Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle 9:1421-33 (2010). PubMed: 20305393
- Sakasai R et al. Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin. DNA Repair (Amst) 9:76-82 (2010). PubMed: 19959400