Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton 20 min at RT
Blocking step
Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit IgG Cy3
Host species: Sheep
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Sheep
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500
Additional data
Abcam response
Thank you for your valuable feedback. For a stronger signal, we would suggest blocking for 1 hour at RT and an overnight incubation at 4ºC with the antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Adam Gracz
Verified customer
提交于 Jan 04 2013