Anti-DLL1抗体(ab10554)
Key features and details
- Rabbit polyclonal to DLL1
- Suitable for: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-DLL1抗体
参阅全部 DLL1 一抗 -
描述
兔多克隆抗体to DLL1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken -
免疫原
Synthetic peptide:
CGEEWSQDLHSSGRTDLRYS
conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. The product is sterile, filtered. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
1/100 - 1/1000. Predicted molecular weight: 78 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/100 - 1/1000. Predicted molecular weight: 78 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. |
靶标
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功能
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
组织特异性
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
序列相似性
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
翻译后修饰
Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 28514 Human
- Entrez Gene: 13388 Mouse
- Entrez Gene: 84010 Rat
- Omim: 606582 Human
- SwissProt: O00548 Human
- SwissProt: Q61483 Mouse
- SwissProt: P97677 Rat
- Unigene: 379912 Human
see all -
别名
- Delta (Drosophila) like 1 antibody
- Delta antibody
- Delta like 1 (Drosophila) antibody
see all
图片
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ab10554 staining DLL1 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 4% BSA for 1 hour. Samples were incubated with primary antibody for 1 hour at room temperature. DAPI used to stain nuclei
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ab10554 staining DLL1 in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (2ug/ml) for 1.5 hours at room temperature. A peroxidase-conjugated polyclonal was used as the secondary antibody. Nuclei stained purple with hematoxylin.
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ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
Lane 2: Mouse Pancreas
Lane 3: Mouse Brain
Lane 4: Rat Heart
Lane 5: Rat Pancreas
Lane 6: Rat BrainSecondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 1 minute
Expected molecular weight: 77kDa
Cell lysates at 20µ g per lane.NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.
Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab10554 被引用在 21 文献中.
- Liu CF et al. Whole-Transcriptome Profiling of Human Heart Tissues Reveals the Potential Novel Players and Regulatory Networks in Different Cardiomyopathy Subtypes of Heart Failure. Circ Genom Precis Med 14:e003142 (2021). PubMed: 33517678
- Zhang Q et al. Single-cell analysis reveals dynamic changes of neural cells in developing human spinal cord. EMBO Rep 22:e52728 (2021). PubMed: 34605607
- Marchetti M et al. MicroRNA-24-3p Targets Notch and Other Vascular Morphogens to Regulate Post-ischemic Microvascular Responses in Limb Muscles. Int J Mol Sci 21:N/A (2020). PubMed: 32138369
- Chang W et al. Hormonal Suppression of Stem Cells Inhibits Symmetric Cell Division and Gastric Tumorigenesis. Cell Stem Cell 26:739-754.e8 (2020). PubMed: 32142681
- Wang DP et al. microRNA-599 promotes apoptosis and represses proliferation and epithelial-mesenchymal transition of papillary thyroid carcinoma cells via downregulation of Hey2-depentent Notch signaling pathway. J Cell Physiol 235:2492-2505 (2020). PubMed: 31565805