Anti-DLK-1抗体[MM0514-9D8] (ab89908)
Key features and details
- Mouse monoclonal [MM0514-9D8] to DLK-1
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-DLK-1抗体[MM0514-9D8]
参阅全部 DLK-1 一抗 -
描述
小鼠单克隆抗体[MM0514-9D8] to DLK-1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human DLK-1.
Database link: P80370 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered. -
克隆
单克隆 -
克隆编号
MM0514-9D8 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89908于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
Use 2µg for 106 cells.
We recommend to fix with 4% formaldehyde for 10min at room temperature.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 2µg for 106 cells. We recommend to fix with 4% formaldehyde for 10min at room temperature.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
May have a role in neuroendocrine differentiation. -
组织特异性
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
序列相似性
Contains 6 EGF-like domains. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8788 Human
- Omim: 176290 Human
- SwissProt: P80370 Human
- Unigene: 533717 Human
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别名
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
图片
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ICC/IF image of ab89908 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89908, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HepG2 cells stained with ab89908 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89908, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
IHC image of ab89908 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89908, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab89908 被引用在 2 文献中.
- Guye P et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . PubMed: 26732624
- Yong KS et al. Human CD34(lo)CD133(lo) fetal liver cells support the expansion of human CD34(hi)CD133(hi) hematopoietic stem cells. Cell Mol Immunol 13:605-14 (2016). ICC/IF . PubMed: 27593483